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The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR.
The HSQC (Heteronuclear Single Quantum Coherence) experiment is used to determine proton-carbon single bond correlations, where the protons lie along the observed F2 (X) axis and the carbons are along the F1 (Y) axis. An edited HSQC spectrum of sucrose at 500 MHz is shown below.
11 lut 2023 · Heteronculear 2-D NMR is the correlation between different nuclei, such as a 1 H to 13 C and heteronuclear 2-D NMR is especially important in biological chemistry, especially in the elucidation of the three-dimensional structure of proteins.
Heteronuclear Single Quantum Coherence (HSQC) is a technique used in NMR spectroscopy to simultaneously record 1H and 13C chemical shifts by coupling 1H nuclei with other NMR-active nuclei directly bonded to them, providing structural information in complex mixtures.
2 kwi 2018 · Nuclear Magnetic Resonance (NMR) spectroscopy is an incredibly powerful tool for characterizing molecular structures. When submitting to the FDA or other regulatory agencies, full structural characterization by NMR provides crucial evidence of compound identity.
24 lip 2019 · Heteronuclear Single Quantum Coherence (HSQC) A protein is make-up of a series of amino acid monomers. Although there are 19 different amino acids each with a distinct side chain, the protein backbone is an invariable pattern of NH-C-CO as shown in Figure 5. Figure 5: Structure of a amino acid chain with NMR active nuclei
HSQC - Heteronuclear Single Quantum Coherence. Term HSQC is also frequently used to describe 2D NMR experiment correlating chemical shifts of nuclei of two different atoms (one type of atom in dimension ω1 and another type of atom in ω2).
