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Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first- strand cDNA from a total RNA preparation.
The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly (A) + or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system and the amount of starting material can vary from 1 pg to 5 μg of total RNA.
The InvitrogenTM SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT–PCR.
The Invitrogen ™ SuperScript III First–Strand Synthesis SuperMix is an optimized SuperMix formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.
SuperScript™ III Reverse Transcriptase, with reduced RNase H activity and higher thermal stability, produces high yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts; Generates high-quality cDNA for use in a variety of applications, including cloning and PCR
SuperScriptTM III Reverse Transcriptase is an engineered version of M-MLV RT with reduced RNase H activity and increased thermal stability. The enzyme is purified to near homogeneity from E. coli containing the modified pol gene of Moloney Murine Leukemia Virus (1,2).
SuperScriptTM III Reverse Transcriptase is a version of M–MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45−60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.
