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“Tissue processing” describes the steps required to take an animal or human tissue from fixation to the state where it is completely infiltrated with a suitable histological wax and can be embedded ready for section cutting on the microtome.
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The preparation of a tissue specimen for microscopic evaluation typically takes between 12 to 48 hours. This process includes fixation, processing, embedding, sectioning, and staining, with each step requiring specific durations to ensure tissue integrity and diagnostic quality.
The techniques for processing the tissues, whether biopsies, larger specimens removed at surgery, or tissues from autopsy, are described below. The persons who do the tissue processing and make the glass microscopic slides are histotechnologists.
14 gru 2018 · An important first step in the histological process is tissue acquisition. This step can be achieved by means of traditional tissue dissection or endoscopic ultrasound (EUS)-guided fine needle aspiration [4].
1 sty 2010 · Histological procedures aim to provide good quality sections that can be used for a light microscopic evaluation of human or animal tissue changes in either spontaneous or induced diseases.
There are three main stages involved in tissue processing. These are Fixation to make sure tissue is fixed properly in the required fixative, Dehydration via ethanol to remove any water in the tissue, Clearing to replace any water or ethanol with xylene, and finally Impregnation with molten wax, which are described on the chart below.
3 gru 2021 · Tissue processing is the technique by which fixed tissues are made suitable for embedding within a supportive medium such as paraffin, and consists of three sequential steps: dehydration, clearing, and infiltration.
