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1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Primer-blast tries to find target-specific primers by placing candidate primers on unique template regions that are not similar to other targets.

   • Tips for Finding Specific Primers

     Try "re-search for specific primers" feature on Primer-BLAST...

   • Publication

     Primer-BLAST was developed at NCBI to help users make...

   • Retrieve Recent Results

     Retrieve recent primer-blast jobs. Enter a job id: Last...

   • Primer-BLAST Help

     Primer-BLAST was developed at NCBI to help users make...

   • Practice Primer-BLAST Examples

     We'll use Primer-BLAST to design primers for: A human...

   • What is Primer-BLAST?

     Primer-BLAST is a combination of Primer3, a popular primer...

2. www.ncbi.nlm.nih.gov › guide › howtoDesign PCR primers and check them for specificity - National...

   www.ncbi.nlm.nih.gov › guide › howto

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   Primer BLAST performs only a specificity check when a target template and both primers are provided. In the Primer Pair Specificity Checking Parameters section, select the appropriate source Organism and the smallest Database that is likely to contain the target sequence.

3. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer-Blast results - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.

4. www.nlm.nih.gov › ncbi › workshopsPractice Primer-BLAST Examples - National Library of Medicine

   www.nlm.nih.gov › ncbi › workshops

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   Learn how to use Primer-BLAST to design primers for different purposes and scenarios, such as human transcripts, SNPs, and cross-species primers. Follow the step-by-step instructions and screenshots for each example and check your results.

5. pmc.ncbi.nlm.nih.gov › articles › PMC3412702Primer-BLAST: A tool to design target-specific primers for...

   pmc.ncbi.nlm.nih.gov › articles › PMC3412702

   Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.

6. www.nlm.nih.gov › ncbi › workshopsWhat is Primer-BLAST? - National Library of Medicine

   www.nlm.nih.gov › ncbi › workshops

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   14 paź 2022 · Primer-BLAST combines Primer3 and NCBI BLAST to design primers and identify targets for PCR amplification. It can check primer and template Tm, secondary structure, self-complementarity, and potential off-target matches.

7. www.nlm.nih.gov › ncbi › workshopsWhat is Primer-BLAST? - National Library of Medicine

   www.nlm.nih.gov › ncbi › workshops

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   22 wrz 2023 · Primer-BLAST is a combination of Primer3, a popular primer designing tool, and NCBI BLAST. Primer-BLAST has two basic modes. Design primers to specifically amplify a template sequence or a set of related templates from a background nucleic acid population (BLAST database).