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1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Primer-blast tries to find target-specific primers by placing candidate primers on unique template regions that are not similar to other targets.

   • Tips for Finding Specific Primers

     Allow primers to amplify mRNA splice variants. This will...

   • Publication

     Primer-BLAST was developed at NCBI to help users make...

   • Retrieve Recent Results

     Retrieve recent primer-blast jobs. Enter a job id: Last...

   • Practice Primer-BLAST Examples

     Practice Primer-BLAST Examples We'll cover three main...

   • Primer-BLAST Help

     Primer-BLAST was developed at NCBI to help users make...

   • The Primer-BLAST interface

     The Primer-BLAST interface. The Primer-BLAST input form has...

   • What is Primer-BLAST?

     Primer-BLAST is a combination of Primer3, a popular primer...

   • Help

     Primer-blast tries to find target-specific primers by...

2. www.ncbi.nlm.nih.gov › guide › howtoDesign PCR primers and check them for specificity - National...

   www.ncbi.nlm.nih.gov › guide › howto

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   Go to the Primer BLAST submission form. Enter the target sequence in FASTA format or an accession number of an NCBI nucleotide sequence in the PCR Template section of the form. If the NCBI mRNA reference sequence accession number is used, the tool will automatically design primers that are specific to that splice variant.

3. www.nlm.nih.gov › ncbi › workshopsPractice Primer-BLAST Examples - National Library of Medicine

   www.nlm.nih.gov › ncbi › workshops

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   Learn how to use Primer-BLAST to design primers for human, whale and other sequences. Follow the steps and screenshots for different scenarios and options.

4. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer-Blast results - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.

5. www.nlm.nih.gov › ncbi › workshopsThe Primer-BLAST interface - National Library of Medicine

   www.nlm.nih.gov › ncbi › workshops

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   16 paź 2022 · Learn how to use the Primer-BLAST tool to design and check PCR primers for various templates and databases. The web page explains the input form sections, parameters, and examples for primer design and specificity checking.

6. www.nlm.nih.gov › ncbi › workshopsWhat is Primer-BLAST? - National Library of Medicine

   www.nlm.nih.gov › ncbi › workshops

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   22 wrz 2023 · Primer-BLAST combines Primer3 and NCBI BLAST to design primers and identify targets for PCR and sequencing. Learn how to use Primer-BLAST modes, features, and options in this workshop.

7. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Allow primers to amplify mRNA splice variants. This will make it much easier to find gene-specific primers if you do not need to distinguish between splice variants. Specify an organism for database search if you are only amplifying DNA from a specific organism.