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8 lut 2022 · Hence, a suitable extraction method that isolates representative samples of glycogen is crucial to the study of this macromolecule. Compared to other extraction methods, a method that employs a sucrose density gradient centrifugation step can minimize molecular damage.
1 cze 2021 · One milder extraction method that employs cold trichloroacetic acid (TCA) has also been used to isolate glycogen (Stetten, Katzen, & Stetten, 1956), with the intention of extracting molecules with less degradation than the hot alkaline method.
1 gru 2007 · The current study was performed to develop convenient, rapid, reliable, and pragmatic methodologies by which to harvest and preserve liver tissue glycogen and to analyze its levels within reasonable limits of quantification and with extended chromophore stability.
1 gru 1981 · The glycogen levels in homogenates from livers from 3- to 4-week-old broilers were measured using alkaline digestion and ethanol precipitation followed by direct hydrolysis with amyloglucosidase. Glycogen was determined with glucose oxidase.
liver and carcass glycogen can produce both energy and gluconeogenic precursors, such as lactate and pyruvate, for the newly hatched chick (Rosebrough et al., 1979).
Methicillin-resistant Staphylococcus aureus (MRSA) isolated from chicken meat and giblets often produces staphylococcal enterotoxin B (SEB) in non- refrigerated raw chicken livers
The document describes isolating glycogen from chicken liver and characterizing carbohydrates through qualitative tests. Glycogen was extracted from chicken liver through grinding, heating, filtration, and precipitation. Tests like Molisch's and iodine reaction confirmed the presence of glycogen.
