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12 lut 2021 · Real-Time RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a sensitive and fast test used for detecting the presence of specific genetic materials within a sample. This genetic material can be specific to humans, bacteria, and viruses like SARS-CoV-2.
- Toby Le
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- Toby Le
12 maj 2020 · Interpreting the result of a test for covid-19 depends on two things: the accuracy of the test, and the pre-test probability or estimated risk of disease before testing. A positive RT-PCR test for covid-19 test has more weight than a negative test because of the test’s high specificity but moderate sensitivity.
SARS-CoV-2 reverse transcriptase–polymerase chain reaction (RT-PCR) testing is highly sensitive (99%) but less specific (approximately 95%) for contagious COVID-19 and may be positive for ≥90 days after infection. Viral antigen tests have lower analytic sensitivity than RT-PCR but are more specific for contagious disease
15 kwi 2021 · Molecular and antigen tests can detect current SARS-CoV-2 infection and are used to diagnose COVID-19 (Table 1). 8, 9 Molecular tests, such as reverse transcriptase polymerase chain reaction...
24 sie 2021 · The polymerase chain reaction (PCR) test for COVID-19 is a molecular test that analyzes your upper respiratory specimen, looking for genetic material (ribonucleic acid or RNA) of SARS-CoV-2, the virus that causes COVID-19.
information could be useful for interpretation of results in asymptomatic persons. However, especially in the current epidemiological context with low virus circulation and low positivity rate of PCR testing (about 1%), it is not recommended to perform a serology test systematically with a PCR test, not even for asymptomatic people (overshooting).
This Viewpoint describes how to interpret 2 types of diagnostic tests commonly in use for SARS-CoV-2 infections—reverse transcriptase–polymerase chain reaction (RT-PCR) and IgM and IgG enzyme-linked immunosorbent assay (ELISA)—and how the results may vary over time .