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  1. PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.

  2. Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .

  3. Composition and preparation of Phosphate Buffered Saline (PBS) is explained in step-by-step. PBS is used as base component for many other buffers like PBS-T, 4 % PFA etc..

  4. Phosphate buffered saline (PBS) is an isotonic solution, unlike water it prevents the rupture of cells or shriveling of cells due to osmosis and is a non-toxic formulation for the cells. As a result, it is widely used in cell culture manufacturing and upstream process development.

  5. PBS is an isotonic buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions. PBS closely mimics the pH, osmolarity, and ion concentrations of the human body. Since it is nontoxic to cells, it.

  6. Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.