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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
PBS is a balanced saline solution that will be used to wash cells to maintain osmotic balance between internal and external environment of cell. From: Methods in Cell Biology, 2023
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
There are many different formulations for phosphate-buffered saline. Some of them contain calcium and magnesium salts. In this page, we are restricting ourselves to provide the most used PBS composition based on (CSHL protocols and Molecular cloning by Sambrook).
Phosphate Buffered Saline (PBS) is a solution prepared by diluting a stock solution of specific salts (NaCl, KCl, Na2HPO4•H2O, KH2PO4) to create a buffer with a pH of 7.3. Tween 20 is added to PBS in concentrations between 0.1% and 0.2% to optimize its performance in biological applications.
Phosphate buffered saline (PBS) is a commonly used solution in biological research and contains NaCl, KCl, KH 2 PO 4 and Na 2 HPO 4 salts. It has been used as a corrosive medium for testing the applicability of Mg alloys for biomedical application [61, 85–88].
28 sty 2019 · PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
