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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
PBS (Phosphate Buffered Saline) (1X, pH 7.4) preparation guide and recipe. Recipe can be automatically scaled by entering desired final volume. PBS is an isotonic buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions.
Composition and preparation of Phosphate Buffered Saline (PBS) is explained in step-by-step. PBS is used as base component for many other buffers like PBS-T, 4 % PFA etc..
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
28 sty 2019 · PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
Measuring analyte concentration using serial dilutions and standard curve. Phosphate buffered saline , more commonly known as PBS, is one of the most commonly used buffers in different biological research applications for maintaining the pH of a solution.
PBS is a balanced saline solution that will be used to wash cells to maintain osmotic balance between internal and external environment of cell. From: Methods in Cell Biology, 2023
