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PBS is a balanced saline solution that will be used to wash cells to maintain osmotic balance between internal and external environment of cell. From: Methods in Cell Biology, 2023
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
Understand the processes of science. Distinguish between scientific and everyday meanings of key words—theory, hypothesis, law, fact—and use in context. Recognize the variables that affect...
The Powerpoint gives a broad overview of some of the important concepts in biology, beginning with the characteristics of life. Each of the levels of biological organization are outlined, from a molecule up through the entire biosphere.
Biology is a science, but what exactly is science? What does the study of biology share with other scientific disciplines? Science (from the Latin scientia, meaning “knowledge”) can be defined as knowledge about the natural world.
