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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
Phosphate buffered saline (PBS) is an isotonic solution, unlike water it prevents the rupture of cells or shriveling of cells due to osmosis and is a non-toxic formulation for the cells. As a result, it is widely used in cell culture manufacturing and upstream process development.
PBS is a balanced saline solution that will be used to wash cells to maintain osmotic balance between internal and external environment of cell. From: Methods in Cell Biology, 2023
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
28 sty 2019 · PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
8 lip 2019 · PBS and dPBS are the abbreviations of phosphate-buffered saline and Dulbecco’s phosphate-buffered saline, respectively. They are well-known buffer solutions that are commonly employed in biological research to maintain a consistent pH (between 7.2-7.6).
While PBS serves as a versatile buffer solution suitable for a myriad of applications, DPBS offers enhanced formulation tailored specifically for cell culture. Understanding the differences between these solutions and their respective applications empowers researchers to make informed decisions, ensuring optimal experimental outcomes and ...
