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1. Phosphate buffered saline (PBS) is used to clean samples. 2. Buffer A (20 mM tricine and 250 mM sucrose, pH 7.8) is used to protect intracellular organelles in the sample homogenization. 3. Buffer B (20 mM HEPES, 100 mM KCl, and 2 mM MgCl 2, pH 7.4) is used for washing the isolated LDs. 4.
Phosphate-buffered saline. Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. PBS is isotonic and non-toxic to cells. It is used in several applications of tissue culture, most notably for cell washing and trypsinization.
PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, it's isotonic to human solutions, so it's less likely to cause cell damage, toxicity, or unwanted precipitation in biological, medical, or biochemical research.
SHS_Biology 1 - Q1 - Module 3_reviewed_edited - Free download as PDF File (.pdf), Text File (.txt) or read online for free.
Klb Biology Book 3 5th Edition - Free ebook download as PDF File (.pdf) or read book online for free. Scribd is the world's largest social reading and publishing site.
12 mar 2013 · KS3 Science Book 1: Eating, Drinking and Breathing. 193885_P048_085.indd 50. 09/12/2013 15:21. A balanced diet. . 2 .2. Each of the food groups has a different role to play in the balanced diet ...
