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Study with Quizlet and memorize flashcards containing terms like DNA ligase, How does DNA ligase make the DNA continuous?, How are the gaps in DNA bridged together? and more.
- DNA-DNA ligase Flashcards
Origins of replication. Site where the replication of a DNA...
- DNA LIgase Flashcards
Study with Quizlet and memorize flashcards containing terms...
- DNA-DNA ligase Flashcards
Origins of replication. Site where the replication of a DNA molecule begins. Replication fork. A Y shaped region on a replicating DNA molecule where new strands are growing. Study with Quizlet and memorize flashcards containing terms like DNA, Transformation, Bacteriophage and more.
Study with Quizlet and memorize flashcards containing terms like DNA Ligase, DNA polymerase, Topoisomerase and more.
24 paź 2024 · DNA ligase is an essential enzyme that plays a critical role in the integrity and maintenance of DNA within living organisms. Its primary function is to facilitate the joining of DNA strands by catalyzing the formation of phosphodiester bonds, a crucial process in DNA repair, replication, and recombination.
6 wrz 2023 · DNA ligase is an enzyme that catalyzes the formation of a covalent bond between a 5’ phosphate and a 3’ hydroxyl group at the termini of DNA fragments; this bond ligates the two DNA fragments together.
DNA ligase catalyzes a strand-joining reaction at a nick junction formed by the annealing of two DNA strands on a DNA template. The requirement of base-pair complementarity at the nick junction has been explored to develop ligase-based technologies for the detection of sequence variance.
In molecular biology, DNA ligase is extensively used to join together DNA fragments with compatible ends during the cloning process. For example, after a DNA fragment has been inserted into a plasmid vector, DNA ligase is used to seal the insert into the plasmid, creating a stable molecule that can be introduced into bacteria for amplification ...