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By fragmenting DNA molecules and analyzing the resulting mass spectra, researchers can pinpoint uracil with high precision. This approach is valuable for its ability to provide quantitative data, offering insights into the extent of uracil incorporation across different samples.
22 wrz 2010 · Uracil may occur in DNA due to either cytosine deamination or thymine replacing incorporation. Its quantitative characterization is important in assessing DNA damages in cells with perturbed thymidylate metabolism or within different DNA segments involved in immunoglobulin gene diversification.
28 wrz 2010 · The finding that extra-helical U can develop spontaneously from an intermediate open U–A base pairs combined to the U–A versus T–A sequence dependence of base pair opening kinetics derived from NMR experiments, has important implications in the repair efficiency shown by UDGs.
Uracil (U) can be found in DNA as a mismatch paired either to adenine (A) or to guanine (G). Removal of U from DNA is performed by a class of enzymes known as uracil-DNA-glycosylases (UDG). Recent studies suggest that recognition of U-A and U-G mismatches by UDG takes place via an extra-helical mech …
10 sie 2020 · Uracil may arise in DNA as a result of spontaneous cytosine deamination and/or misincorporation of dUMP during DNA replication. In this paper we will review: (i) sources of the origin of uracil in DNA; (ii) some properties of the enzymes responsible for the excision of uracil and their role in the I ….
1 gru 2010 · Based on the literature data we conclude/hypothesize that the non-canonical base uracil may be present and well tolerated in DNA mostly as U:A pairs, likely in quantities of 10 4 per genome.
1 paź 2015 · Genomic uracil is specifically recognized by representatives of the uracil-DNA glycosylase superfamily (UDG), cleaving the N-glycosidic bond between the pyrimidine ring and deoxyribose and resulting in apyrimidinic (AP) sites that are further processed by base excision repair (8, 9).
