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  1. To prepare the urea base, dissolve the first six ingredients in 100 ml of distilled water and filter sterilize (0.45-mm pore size). Suspend the agar in 900 ml of distilled water, boil to dissolve completely, and autoclave at 121oC and 15 psi for 15 minutes. Cool the agar to 50 to 55oC.

  2. In Rapid Urease test Broth the urease reaction given by H. pylori, occurs more quickly than that seen by other organisms which may split urea. As a result, it is an effective presumptive test for the presence of H. pylori.

  3. Urea Broth Base (Dehydrated) is a differential media used to differentiate Enterobacteriaceae isolates by urease production from clinical samples (Stool and urine samples). The devices are for professional use only and is not intended for self-testing.

  4. INTENDED USE. Remel Rapid Urea Broth (Lyophilized) is recommended for use in qualitative procedures for rapid differentiation of microorganisms on the basis of urease production. SUMMARY AND EXPLANATION. The detection of urease activity is routinely used in clinical microbiology laboratories to aid in identification of gram-negative bacteria.

  5. Urea Broth Base (Dehydrated) is a differential media used to differentiate Enterobacteriaceae isolates by urease production from clinical samples (Stool and urine samples).

  6. INTENDED USE. Remel Urea Broth (Stuart’s) is a liquid medium recommended for use in qualitative procedures to aid in differentiation of genera within the Enterobacteriaceae on the basis of urease production.

  7. Directions: Dissolve 38.7 g in 1 litre distilled water and filter sterilize. This medium must NOT be heated. Principle and Interpretation: Urea Broth is a highly buffered urea medium prepared according to the formula of Stuart, Van Stratum and Rustigian (2).

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