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powiązane z: universal primer pcr protocolChoose from our dual-labeled TaqMan probes & unlabeled oligos for use as qPCR primers. Explore our portfolio of unlabeled oligos, dyes and quenchers for qPCR assay design.
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1 lip 1997 · We describe the development and application of a universal method for the labelling of any PCR product using a single end-labelled primer. Amplification was performed in a single reaction with the resulting amplicon labelled to a high specific activity.
11 gru 2018 · To equip designed primers with a universal tail and optimize a single-tube PCR protocol to achieve sequencing results comparable to commercially available kits such as the Nextera indexing kit.
Benefits of a universal annealing temperature for PCR primers. Learn the importance of the annealing step in PCR, how to circumvent optimization steps using a specially formulated PCR buffer, and the benefits of a universal annealing temperature enabled by the buffer.
17 sty 2012 · Universal Primer-Multiplex PCR (UP-M-PCR) Compared with traditional multiplex PCR system, UP-M-PCR contains more of a universal primer (UP) at a normal concentration (500 nmol/L), a little quantity (10∼50 nmol/L) of 15 compound specific primers targeting for the 15 marker and endogenous genes.
5 lip 2007 · Here, we present an alternative to the standard labeling of PCR products for analysis by Pyrosequencing that circumvents the requirement of specific biotinylated primers for each SNP of...
17 sty 2012 · In the present study, we also use this novel universal primer-multiplex PCR method to simultaneously detect nine commonly used selectable marker and reporter genes, including hpt, gus, nptII, aadA, pat, 35s, bar, nos, uidA, as well as six endogenous genes Pa, Ivr, Lec, sps, sad1 and FatA of six most common GM crops papaya, maize, soybean, rice, ...
13 paź 2023 · In this study, we developed a primer design program called UPrimer that can quickly design clade-specific NPCL amplification primers based on genome data, without requiring manual intervention.
Reklama
powiązane z: universal primer pcr protocolChoose from our dual-labeled TaqMan probes & unlabeled oligos for use as qPCR primers. Explore our portfolio of unlabeled oligos, dyes and quenchers for qPCR assay design.
