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1 gru 2010 · Objective —To evaluate the usefulness of a PCR-based telomeric repeat amplification protocol (TRAP) assay for detecting telomerase activity in cells from a canine transitional cell carcinoma (TCC) cell line and, ultimately, in the urine of dogs with TCC.
The assay has recently been modified to allow for the quantitative assessment of telomerase using enzyme-linked immunosorbent assay technology. Studies using the TRAP assay have shown that expression of telomerase is strictly regulated, at least in human tissues.
The TRAP assay can be used to measure telomerase activity in malignant tumors of dogs. Because telomerase activation may be required for indefinite longevity of cells, it may also serve...
20 lis 2015 · The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
In this detailed protocol we describe Telomere Repeat Amplification Protocol (TRAP). The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
The telomerized clones show substantial telomerase activity (as measured by TRAP assay (Fig. 3C) and maintain stable telomere lengths (6–8 kb) compared to non-telomerized, senescent melanocytes which show an average telomere length of ∼4–6 kb (Fig. 3D).
The TRAPEZE® Gel-Based Telomerase Detection Kit provides substantial improvements to the original TRAP assay, such as a modified reverse primer sequence which (1) eliminates the need for a wax barrier hot start, (2) reduces amplification artifacts and (3) permits better estimation of telomerase processivity.
