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The Telomeric repeat amplification protocol (TRAP) is a highly sensitive PCR-based assay and prove to be an important tool for understanding the role of telomerase in cancer and various tissues that harbors telomerase positive stem cells.
1 gru 2010 · To evaluate the usefulness of a PCR-based telomeric repeat amplification protocol (TRAP) assay for detecting telomerase activity in cells from a canine transitional cell carcinoma (TCC)...
The assay has recently been modified to allow for the quantitative assessment of telomerase using enzyme-linked immunosorbent assay technology. Studies using the TRAP assay have shown that expression of telomerase is strictly regulated, at least in human tissues.
1 gru 2010 · Objective —To evaluate the usefulness of a PCR-based telomeric repeat amplification protocol (TRAP) assay for detecting telomerase activity in cells from a canine transitional cell carcinoma (TCC) cell line and, ultimately, in the urine of dogs with TCC.
The TRAPEZE® Gel-Based Telomerase Detection Kit provides substantial improvements to the original TRAP assay, such as a modified reverse primer sequence which (1) eliminates the need for a wax barrier hot start, (2) reduces amplification artifacts and (3) permits better estimation of telomerase processivity.
The aim of the study was to evaluate the TRAP assay method of measuring telomerase activity in canine skin neoplasms. Telomerase activity was measured in 57 samples of tumor tissues from...
1 lip 1997 · The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples.
