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The TRAPeze Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
The TRAPeze XL Telomerase Detection Kit is a homogenous fluorescence assay for rapid and sensitive telomerase activity measurement in cell and tissue extracts. The TRAPeze XL Kit incorporates the use of the novel Amplifluor fluorescence energy transfer-labeled primers into the original TRAPeze Telomerase Detection Kit reaction so that ...
TRAP assay performed in dogs has high repetition rate within high activity, and lower within small activity. This method is 100% sensitive and 34% specific. No full-text available. To read...
1 gru 2010 · Objective —To evaluate the usefulness of a PCR-based telomeric repeat amplification protocol (TRAP) assay for detecting telomerase activity in cells from a canine transitional cell carcinoma (TCC) cell line and, ultimately, in the urine of dogs with TCC.
The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
The assay has recently been modified to allow for the quantitative assessment of telomerase using enzyme-linked immunosorbent assay technology. Studies using the TRAP assay have shown that expression of telomerase is strictly regulated, at least in human tissues.
The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.