Search results
The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
1 lip 1997 · The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples.
The current chapter describes various TRAP methods to detect telomerase activity (TA) using gel-based methods, its advantages and deficits, how to perform an ELISA-based TRAP assay and how best to interpret its results.
1 sty 2002 · Using the TRAP assay, researchers have measured telomerase activity in ascites, pleural effusions, bronchial lavage and washings, urine, bladder washings, and oral rinses. In all cases but the last, the TRAP assay was more sensitive than standard cytology in identifying patients with cancer.
20 lis 2015 · The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
1 sty 2024 · The current chapter describes various TRAP methods to detect telomerase activity (TA) using gel-based methods, its advantages and deficits, how to perform an ELISA-based TRAP assay and how best to interpret its results.
The polymerase chain reaction (PCR) based telomeric repeat amplification protocol (TRAP) assay, to detect telomerase activity was originated by Kim et al. in late 1994 (1) and revolutionized the field of telomere/telomerase research in aging, but particularly in cancer.
