Search results
In this detailed protocol we describe Telomere Repeat Amplification Protocol (TRAP). The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
1 lip 1997 · The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples.
The development of the TRAP assay provided initially a highly sensitive method to measure telomerase activity owing to signal amplification by PCR and improved detergent lysis to allow more uniform extraction of telomerase from a small number of cells (1,11,12).
22 mar 2017 · The activity of the telomerase may serve as a diagnostic marker for malignancy, and an indicator of the proliferative potential of somatic and stem cells. The telomeric repeat amplification protocol (TRAP) is a sensitive and accurate PCR-based assay for telomerase detection and measurement.
1 sty 2024 · The current chapter describes various TRAP methods to detect telomerase activity (TA) using gel-based methods, its advantages and deficits, how to perform an ELISA-based TRAP assay and how best to interpret its results.
The current chapter describes various TRAP methods to detect telomerase activity (TA) using gel-based methods, its advantages and deficits, how to perform an ELISA-based TRAP assay and how best to interpret its results.
The telomeric repeat amplification protocol (TRAP) assay, first published by Kim et al. in 1994 (1), is used to detect telomerase activity. The scope of the technique is far reaching and has implications for telomere, telomerase, aging, and cancer research (2,3).
