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The kit is approved for human In Vitro Diagnostic Use and was primarily intended for detecting TRAP positive cells in peripheral blood or bone marrow, particularly for the detection of TRAP positive cells associated with Hairy Cell Leukemia.
TRAP assay is a popular method to determine telomerase activity. It includes extension, amplification, and detection of telomerase products. This technique is crucial for studying telomerase activity in various cells, tissues, and specimens, especially in the context of cancer research and aging.
1 lut 2021 · In the present study, we aimed to standardize a simplified telomerase repeat-amplification protocol (TRAP) assay to detect telomerase activity in unstimulated and PHA-stimulated mononuclear cells. METHODS and RESULTS: Our optimized qPCR-based can efficiently evaluate telomerase activity.
The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
Commercially available kits based on the TRAP assay for detection of telomerase activity have now become accessible. The TRAPeze™ Telomerase Detection Kit is distributed by Oncor (Gaithersburg, MD) and provides lysis buffer and most PCR reaction components of the standard TRAP assay .
20 lis 2015 · The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
