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The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer.
The kit is approved for human In Vitro Diagnostic Use and was primarily intended for detecting TRAP positive cells in peripheral blood or bone marrow, particularly for the detection of TRAP positive cells associated with Hairy Cell Leukemia.
20 lis 2015 · The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
1 lut 2021 · In the present study, we aimed to standardize a simplified telomerase repeat-amplification protocol (TRAP) assay to detect telomerase activity in unstimulated and PHA-stimulated mononuclear cells. METHODS and RESULTS: Our optimized qPCR-based can efficiently evaluate telomerase activity.
perform the Telomeric Repeat Amplification Protocol (TRAP) and ELISA assay for non-quantitative detection of telomerase activity in cells and tissues. The novice user is advised to read the entire manual prior to using the
The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
The standard TRAP utilizes a 150-bp ITAS (internal telomerase assay standard) while the TRAP-eze kit utilizes a short standard of 36 bp [55,60,62]. Most inhibitors affect PCR amplification as they inhibit Taq polymerase activity.
