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1 lip 1997 · The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples.
The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer.
Commercially available kits based on the TRAP assay for detection of telomerase activity have now become accessible. The TRAPeze™ Telomerase Detection Kit is distributed by Oncor (Gaithersburg, MD) and provides lysis buffer and most PCR reaction components of the standard TRAP assay ( 1 ).
Scheme for TRAP with hybridization protection assay. TRAP combined with enzyme-linked immunosorbent assay (ELISA) In the TRAP-ELISA method, DNA after the amplification is determined colorimetrically, facilitating the qualitative and semi-quantitative assessment of telomerase activity.
20 lis 2015 · The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPeze® Telomerase Detection Kit (Cat.
15 sty 2002 · Two commercially available TRAP‐based kits (see Materials and Methods) utilize biotinylated primers and enzyme‐linked immunosorbent assays (ELISA) for post‐PCR analysis ( 17). To improve linearity, the TRAP‐ELISAs include competitive internal standards that also control for the presence of PCR inhibitors.
