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The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
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The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPeze® Telomerase Detection Kit (Cat.
The TRAPEZE® Gel-Based Telomerase Detection Kit provides substantial improvements to the original TRAP assay, such as a modified reverse primer sequence which (1) eliminates the need for a wax barrier hot start, (2) reduces amplification artifacts and (3) permits better estimation of telomerase processivity.
Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).
The TRAPEZE® XL Kit is a one buffer, two enzyme system utilizing polymerase chain reaction (PCR) and Amplifluor® primers (34). In the first step of the reaction (Figure 1, line A), the telomerase enzyme adds a number of telomeric repeats (GGTTAG) onto the 3' end of a substrate oligonucleotide (TS).
The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original TRAPeze assay and adds the ability to quantitate telomerase activity using fluorescence energy transfer (ET) primers.
The TRAPEZE® XL Kit is a highly sensitive in vitro assay for the fluorometric detection of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPEZE® Telomerase Detection Kit (Cat #S7700).
