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1 lip 1997 · The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples.
20 lis 2015 · The TRAP assay is a popular method to determine telomerase activity in mammalian cells and tissue samples (Kim et al., 1994). The TRAP assay includes three steps: extension, amplification, and detection of telomerase products.
Scheme for TRAP with hybridization protection assay. TRAP combined with enzyme-linked immunosorbent assay (ELISA) In the TRAP-ELISA method, DNA after the amplification is determined colorimetrically, facilitating the qualitative and semi-quantitative assessment of telomerase activity.
The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPeze® Telomerase Detection Kit (Cat.
Telomeric repeat amplification protocol (TRAP) is a fast and sensitive PCR-based assay for detection and measurement of telomerase activity. Since its introduction, the TRAP assay has been widely used in cancer and aging studies.
The polymerase chain reaction (PCR) based telomeric repeat amplification protocol (TRAP) assay, to detect telomerase activity was originated by Kim et al. in late ...
The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
