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The TRAPeze® RT Telomerase Detection Kit is a highly sensitive in vitro assay for the fluorometric detection and real time quantification of telomerase activity. It incorporates refinements to the original TRAP assay that were first introduced in the gel-based TRAPeze® Telomerase Detection Kit (Cat.
The TRAPEZE® Gel-Based Telomerase Detection Kit provides substantial improvements to the original TRAP assay, such as a modified reverse primer sequence which (1) eliminates the need for a wax barrier hot start, (2) reduces amplification artifacts and (3) permits better estimation of telomerase processivity.
Telomeric repeat amplification protocol (TRAP)—a sensitive, PCR-based assay to detect telomerase activity was quintessential to the evaluation of telomerase role in telomere maintenance, cell proliferation, tumour development, and cell immortalization.
The TRAPEZE® XL Kit is a one buffer, two enzyme system utilizing polymerase chain reaction (PCR) and Amplifluor® primers (34). In the first step of the reaction (Figure 1, line A), the telomerase enzyme adds a number of telomeric repeats (GGTTAG) onto the 3' end of a substrate oligonucleotide (TS).
perform the Telomeric Repeat Amplification Protocol (TRAP) and ELISA assay for non-quantitative detection of telomerase activity in cells and tissues. The novice user is advised to read the entire manual prior to using the
The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
The kit is approved for human In Vitro Diagnostic Use and was primarily intended for detecting TRAP positive cells in peripheral blood or bone marrow, particularly for the detection of TRAP positive cells associated with Hairy Cell Leukemia.
