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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
TmProt is a web tool for sequence-based prediction of protein melting temperatures. Despite recent progress in high-throughput protein characterization, protein databases remain largely unannotated, presenting ample opportunities for discovering promising biocatalysts using automated tools like EnzymeMiner .
This tool was created to aid in the prediction of melting temperatures for primers, probes, and small oligos. Disclaimer: Access to software and related materials are provided as a courtesy, free of charge, and are intended for informational and educational purposes only.
Tm Predictor P.C. Lyu Lab., Institute of Bioinformatics and Structural Biology, National Tsing-Hua University, Hsin-Chu, Taiwan . Please Input the Protein Sequence :(plain text) Melting Temperature Prediction. Tm Index . Predited Tm >1 >65℃ <0 <55℃ 0~1. 55℃~65℃ Melting Temperature Index (TI) Mail to ...
This tool predicts the melting temperature (Tm) of oligonucleotides enhanced with locked nucleic acids (LNA)
ProTDet is a machine learning tool designed to predict the melting temperature of proteins based on their sequence. It simplifies the process of accessing the thermostability of native proteins and provides an efficient solution for protein designers.
The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient.
