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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
ProTDet is a machine learning tool designed to predict the melting temperature of proteins based on their sequence. It simplifies the process of accessing the thermostability of native proteins and provides an efficient solution for protein designers.
Tm Predictor P.C. Lyu Lab., Institute of Bioinformatics and Structural Biology, National Tsing-Hua University, Hsin-Chu, Taiwan . Please Input the Protein Sequence :(plain text) Melting Temperature Prediction. Tm Index . Predited Tm >1 >65℃ <0 <55℃ 0~1. 55℃~65℃ Melting Temperature Index (TI) Mail to ...
Select ANALYZE for easy, one-click access to a T m calculator, GC content calculator, extinction coefficient calculator, and more. Predict oligo secondary structure or possible duplexes with HAIRPIN, SELF-DIMER, and HETERO-DIMER options.
Protein calculator (C. Putnam, The Scripps Research Institute, U.S.A.) - calculates mass, pI, charge at a given pH, counts amino acid residues etc. Tm Predictor (P.C. Lyu Lab., National Tsing-Hua University, Taiwan) - calculates the theoretical protein melting temperature.
This tool was created to aid in the prediction of melting temperatures for primers, probes, and small oligos. As a beta version, feedback is always welcome and users can contact us zach.dwight [at] path.utah.edu.
This tool predicts the melting temperature (Tm) of oligonucleotides enhanced with locked nucleic acids (LNA)
