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The TRAPEZE® Gel-Based Telomerase Detection Kit provides substantial improvements to the original TRAP assay, such as a modified reverse primer sequence which (1) eliminates the need for a wax barrier hot start, (2) reduces amplification artifacts and (3) permits better estimation of telomerase processivity.
The present study looked at whether telomerase activity might serve as a marker for canine tumors. Telomerase activity was measured using the telomeric repeat amplification protocol assay. Normal dog somatic tissues showed little or no telomerase activity, while normal testis exhibited a high level of telomerase activity. We measured telomerase ...
9 lis 2000 · This paper provides the first comprehensive study of telomerase activity in normal somatic tissues and demonstrates that the distribution of telomerase activity among tissue in dogs is similar to humans, i.e., germinal tissues and tumour tissues have significant telomerase, whereas other somatic tissues show low or no activity.
1 lut 2008 · The most commonly employed method to detect telomerase activity in dogs is the telomere repeat amplification protocol (TRAP) (Kim et al., 1994). This assay has the advantage that it allows detection of active holoenzyme in cell extracts and can be performed quantitatively using commercially available kits but requires good quality tissue ...
5 maj 2019 · Peroxidase activity was detected using a chemiluminescence ECL HRP substrate reagent kit (GE Healthcare, Buckinghamshire, UK).
Telomerase Activity Quantification qPCR Assay Kit (TAQ) is designed to quantitatively compare telomerase activity among cell populations. The cell lysis buffer is a mild lysis buffer that enables the release of telomerase in the native state.
1 lut 2008 · Overall, cats and dogs seem to regulate telomerase activity in a manner similar to humans, and are likely to utilize replicative senescence as an anticancer mechanism. ...
