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2 maj 2024 · In this experiment, pigments are extracted from spinach leaf and analyzed and separated by thin layer chromatography (TLC) and column chromatography (CC). Spinach chromatography allows the separation of substances from a mixture based on the different affinity that these products have for the stationary phase (silica gel) and a mobile phase ...
Period 1: Isolation of Pigment from Spinach Leaves. Weigh about 1.0 g of fresh spinach leaves (avoid using stems or thick veins). Cut or tear the spinach leaves into small pieces and place them in a mortar along with 0.5 g of anhydrous sodium sulfate (Na2SO4) and 2.0 mL of acetone.
An example is the spinach leaf, which appears dark green, but is actually a mixture of several pigments of different color. In order to identify the individual pigments, chemists use a technique known as chromatography (‘chroma’ refers to color) to separate out the different pigments.
30 cze 2024 · The overall results indicated that hyperspectral imaging with multi-task learning could predict the quality attributes of spinach simultaneously for spinach quality inspection under various storage conditions.
30 gru 2004 · Separation and Determination of Chloroplast Pigments from Spinach by Thin-Layer Chromatography: a Student Laboratory Experiment. Practice of Planar Chromatography. Published: 30 December 2004. Volume 17, pages 309–313, (2004) Cite this article. Download PDF. Joseph Sherma & Bernard Fried. 312 Accesses. 8 Citations. Explore all metrics. Article PDF.
Learning Objectives. After completing the lab, the student will be able to: Extract pigments from plant material. Separate pigments by paper chromatography. Activity 1: Pre-Assessment. Think about what colors you have seen in leaves of plants. What determines the color of a leaf?
Step 1: Hypothesize/Predict: Discuss with your lab partner what color pigments will likely be present in the spinach leaves. Write your predictions in your lab notebook and draw a diagram of how you think the pigments will separate out on the chromatography paper. Step 2: Student-led Planning: Read step 3 below. Discuss with your lab partner ...
