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2 maj 2024 · In this experiment, pigments are extracted from spinach leaf and analyzed and separated by thin layer chromatography (TLC) and column chromatography (CC). Spinach chromatography allows the separation of substances from a mixture based on the different affinity that these products have for the stationary phase (silica gel) and a mobile phase ...
Period 1: Isolation of Pigment from Spinach Leaves. Weigh about 1.0 g of fresh spinach leaves (avoid using stems or thick veins). Cut or tear the spinach leaves into small pieces and place them in a mortar along with 0.5 g of anhydrous sodium sulfate (Na2SO4) and 2.0 mL of acetone.
Chromatography is an analytical method permitting the separation of a mixture into its molecular components. In this technique, a concentrated spot of the pigment mixture is deposited at one end of a paper strip. The paper strip is called the stationary phase.
Plant Pigment Chromatography. Students will isolate and identify photosynthetic pigments in spinach leaves. Students will calculate Rf values of photosynthetic pigments and graph the absorption spectrum for each pigment.
Experiment 3 – TLC Analysis of Spinach Pigments Learning Objectives • Understand principles behind solid-liquid extraction, liquid-liquid extraction, and thin-layer chromatography (TLC) • Critical analysis of extraction & TLC technique • Analyze data to assess components of extract and success of experiment
15 kwi 2013 · The current experiment emphasizes the green chemical principles of renewable feedstocks and recycling to minimize waste, while simultaneously introducing or reinforcing common organic techniques, including solvent extraction, column chromatography, and thin-layer chromatography for the isolation and identification of photosynthetic pigments ...
Learning Objectives. After completing the lab, the student will be able to: Extract pigments from plant material. Separate pigments by paper chromatography. Activity 1: Pre-Assessment. Think about what colors you have seen in leaves of plants. What determines the color of a leaf?
