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This protocol is applied in the routine staining of cationic and anionic tissue components in tissue sections. This is the standard reference stain used in the study of histochemical tissue pathology.
For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
Hematoxylin and Eosin (H&E) Staining – Manual Protocol (From Baylor College of Medicine) Protocol for H&E staining: •Place slides containing paraffin sections in a slide holder (glass or metal) •Deparaffinize and rehydrate sections: 3 x 3´ Xylene (blot excess xylene before going into ethanol)
Hematoxylin staining: the paraffin sections stained with hematoxylin about 10 minutes (30 ºC), water rinse for 15 minutes, drain the water; Differentiation: put the paraffin slices into 1% hydrochloric acid ethanol differentiation liquid
This procedure establishes a consistent process for preparing H&E slides from frozen or FFPE tissue samples using the automated H&E stainer and coverslip instruments. This
Hematoxylin and Eosin Stain For Fresh Frozen Section (H&E) PROCEDURE: 1. Take slides immediately from freezer and place in cold fixative (10% Neutral Buffered Formalin or 4% PFA) for 10 minutes 2. Rinse slides in 1X PBS, 2 times for 3 minutes each to remove OCT or other tissue embedding compound 3. Rinse in a gentle stream of tap water for 1 ...
To perform an H&E stain, follow the procedure below to re-hydrate, dehydrate and stain the tissue sections. Same solvents can be used for multiple tissue sections but should be changed daily or more often if they become contaminated (strongly colored by the stains). References: 1. Laboratory methods in histotechnology / edited by: Edna B ...
