Search results
21 sty 2022 · First, it creates a 5′-RNA-DNA-3′ hybrid primer which serves as an optimal substrate for elongation by the replicative DNA polymerase. Second, it promotes primer release by primase.
5 wrz 2019 · Replication of eukaryotic nuclear DNA is initiated when DNA polymerase α (Pol α)-primase synthesizes short RNA–DNA primers that are subsequently extended during synthesis of the two DNA strands...
15 mar 2024 · The mechanism by which polymerase α–primase (polα–primase) synthesizes chimeric RNA-DNA primers of defined length and composition, necessary for replication fidelity and genome stability,...
21 mar 2021 · This preference to reprime using dNTPs may facilitate a more efficient restarting of DNA synthesis as high fidelity replicases preferentially copy B-form DNA templates, whereas priming with an RNA polymer produces an RNA–DNA hybrid that is a much poorer A-form substrate.
The primase however is a typical RNA polymerase, capable of initiating polynucleotide synthesis de novo by positioning a complementary ribonucleoside 5'-triphosphate opposite its complementary DNA base. The primase makes an RNA primer that the DNA polymerase can then use for chain extension.
5 maj 2016 · The human primosome, a 340-kilodalton complex of primase and DNA polymerase α (Polα), synthesizes chimeric RNA-DNA primers to be extended by replicative DNA polymerases δ and ϵ. The intricate mechanism of concerted primer synthesis by two catalytic centers was an enigma for over three decades.
7 lip 2023 · Introduction. Before cell division can occur, a cell’s genome must be duplicated. The process of DNA replication typically begins with the synthesis of short RNA primers by specialised replicases called primases and continues with the extension of these primers by DNA polymerases.
