Search results
Remel Simmons Citrate Agar is a solid medium recommended for use in qualitative procedures to differentiate gram-negative bacteria on the basis of citrate utilization. SUMMARY AND EXPLANATION.
Purpose. The citrate test screens a bacterial isolate for the ability to utilize citrate as its carbon and energy source (3, 7). A positive diagnostic test rests on the generation of alkaline by-products of citrate metabolism. The subsequent increase in the pH of the medium is demonstrated by the color change of a pH indicator.
Simmons citrate agar (sodium citrate minimum mineral medium) is used for the differentiation of Gram negative bacilli. It enables the detection of sodium citrate as the sole source of carbon and energy for bacteria. This medium contributes to demonstration of the identification characteristics of Enterobacteriaceae.
Simmons Citrate Agar is used for the differentiation between Enterobacteriaceae and the members of aerogens group on the basis of citrate utilization as sole carbon source. Ammonium dihydrogen phosphate and sodium citrate serve as the sole nitrogen and carbon source respectively. Bromo thymol blue is the pH indicator.
26 sie 2011 · DEFINITION. Medium used for the differentiation of gram negative bacilli. This medium contributes to demonstration of the identification characteristics of Enterobacteriaceae. STANDARDS. FOOD MICROBIOLOGY. • NF ISO 10273 (December 2003): Microbiology - General guidelines for the detection of suspected pathogenic Yersinia enterocolitica.
In this exercise, students will examine the citrate utilization patterns of selected bacterial species. A commercially-available Simmons Citrate Agar medium (Hardy Diagnostics) will be used for this purpose. Figure 1. Simmons citrate test.
A positive reaction is indicated by growth on the slant with an intense blue color (alkaline reaction). A negative reaction is indicated by inhibition to poor growth without change in color (medium remains green).
