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1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Primer-blast tries to find target-specific primers by placing candidate primers on unique template regions that are not similar to other targets.

   • Tips for Finding Specific Primers

     Try "re-search for specific primers" feature on Primer-BLAST...

   • Publication

     Primer-BLAST was developed at NCBI to help users make...

   • PubMed

     Primer-BLAST allows users to design new target-specific...

   • Practice Primer-BLAST Examples

     We'll use Primer-BLAST to design primers for: A human...

2. www.ncbi.nlm.nih.gov › pmc › articlesPrimer-BLAST: A tool to design target-specific primers for...

   www.ncbi.nlm.nih.gov › pmc › articles

   18 cze 2012 · Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.

3. www.nature.com › articles › s41598/023/43825-0A tool to automatically design multiplex PCR primer pairs for...

   www.nature.com › articles › s41598/023/43825-0

   30 wrz 2023 · We developed PMPrimer, a Python-based tool for automated design and evaluation of multiplex PCR primer pairs for specific targets using diverse templates.

4. pubmed.ncbi.nlm.nih.gov › 22708584Primer-BLAST: a tool to design target-specific primers for ... -...

   pubmed.ncbi.nlm.nih.gov › 22708584

   18 cze 2012 · Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.

5. pubmed.ncbi.nlm.nih.gov › 20301004Primer design for RT-PCR - PubMed

   pubmed.ncbi.nlm.nih.gov › 20301004

   Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity.

6. www.nlm.nih.gov › ncbi › workshopsWhat is Primer-BLAST? - National Library of Medicine

   www.nlm.nih.gov › ncbi › workshops

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   22 wrz 2023 · Primer-BLAST is a combination of Primer3, a popular primer designing tool, and NCBI BLAST. Primer-BLAST has two basic modes. Design primers to specifically amplify a template sequence or a set of related templates from a background nucleic acid population (BLAST database).

7. www.nlm.nih.gov › ncbi › workshopsPractice Primer-BLAST Examples - National Library of Medicine

   www.nlm.nih.gov › ncbi › workshops

   • Zapisane w pamięci cache

   We'll use Primer-BLAST to design primers for: A human transcript from the myeloperoxidase (MPO) gene and use the exon/intron selection to make them specific for the transcript. A specific exon of the human MPO gene that contains a clinically signficant SNP. Starting from the nucleotide record.