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1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

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   Primer-BLAST uses Primer3 and BLAST to design primers for PCR based on a template sequence. You can enter your own primers, set parameters, and check primer specificity and melting temperatures.

   • Tips for Finding Specific Primers

     Try "re-search for specific primers" feature on Primer-BLAST...

   • Publication

     Primer-BLAST was developed at NCBI to help users make...

   • Blast

     The Basic Local Alignment Search Tool (BLAST) finds regions...

   • PubMed

     Primer-BLAST allows users to design new target-specific...

   • PMC Free Article

     Primer-BLAST allows users to design new target-specific...

   • Primer-BLAST Help

     Primer-BLAST Results Help. Primer-BLAST was developed at...

   • Primer design for RT-PCR

     Primer design is a crucial initial step in any experiment...

2. blast.ncbi.nlm.nih.gov › BlastBasic Local Alignment Search Tool - BLAST

   blast.ncbi.nlm.nih.gov › Blast

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   The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

3. pubmed.ncbi.nlm.nih.gov › 22708584Primer-BLAST: a tool to design target-specific primers for ... -...

   pubmed.ncbi.nlm.nih.gov › 22708584

   18 cze 2012 · Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.

4. www.ncbi.nlm.nih.gov › pmc › articlesPrimer-BLAST: A tool to design target-specific primers for...

   www.ncbi.nlm.nih.gov › pmc › articles

   18 cze 2012 · Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.

5. pubmed.ncbi.nlm.nih.gov › 20301004Primer design for RT-PCR - PubMed

   pubmed.ncbi.nlm.nih.gov › 20301004

   Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity.

6. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer-Blast results - National Center for Biotechnology...

   www.ncbi.nlm.nih.gov › tools › primer-blast

   • Zapisane w pamięci cache

   Primer-BLAST Results Help. Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target. It uses Primer3 to design PCR primers and then uses BLAST and global alignment algorithm to screen primers against user-selected database in order to avoid primer pairs (all combinations including forward-reverse ...

7. pubmed.ncbi.nlm.nih.gov › 31578684Parameters for Successful PCR Primer Design - PubMed

   pubmed.ncbi.nlm.nih.gov › 31578684

   Primers are critical components of any PCR assay, as they are the main determinants of its specificity, sensitivity, and robustness.