Search results
13 paź 2022 · Sequencing of PAO1 revealed that P. aeruginosa has more regulatory genes than other sequenced bacteria genomes. The bacterium also has higher expression of transcriptional regulators in the LysR, AraC, ECF-σ, and two-component regulator families (Stover et al., 2000).
31 sie 2000 · A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1.
22 cze 2021 · We identified essential genes in LPAO using transposon mutant sequencing (Tn-seq) of pooled mutant libraries grown on LB agar and compared them to those found previously in PAO1 (see Materials and Methods).
12 mar 2024 · The genome sequences of 17 strains isolated from patients with healthcare-associated infections in a Mexican hospital were genomically and phylogenetically analyzed and antibiotic resistance genes, virulence genes, and biofilm formation genes were detected.
The sequence reads were mapped to the PAO1-UW reference sequence (NC_002516) using Illumina's ELAND aligner (efficient large-scale alignment of nucleotide databases). ELAND aligns a large number of short reads to reference sequences, allowing up to two mismatches in the first 32 bases of a read.
Taking the existing PAO1-UW genome sequence (named after the University of Washington, which led the sequencing project) as a blueprint, the genome sequences of reference strains MPAO1 and PAO1-DSM (stored at the German Collection for Microorganisms and Cell Cultures [DSMZ]) were resolved by physical mapping and deep short read sequencing-by ...
21 gru 2022 · Here, we use recently assembled transcriptome compendia of publicly available P. aeruginosa RNA sequencing (RNA-seq) samples to create two smaller compendia consisting of only strain PAO1 or strain PA14 samples with each aligned to their cognate reference genome.
