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13 paź 2022 · Sequencing of PAO1 revealed that P. aeruginosa has more regulatory genes than other sequenced bacteria genomes. The bacterium also has higher expression of transcriptional regulators in the LysR, AraC, ECF-σ, and two-component regulator families (Stover et al., 2000).
22 cze 2021 · We identified essential genes in LPAO using transposon mutant sequencing (Tn-seq) of pooled mutant libraries grown on LB agar and compared them to those found previously in PAO1 (see Materials and Methods).
31 sie 2000 · A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1.
6 sty 2020 · Up to October 2019, the Pseudomonas Genome Database, a database devoted to the information on Pseudomonas species , contained 4660 sequenced P. aeruginosa genomes, including 22 PAO1 sublines.
12 mar 2024 · The pubMLST database was used to determine the multilocus sequence type, selecting Pseudomonas aeruginosa as an organism, single sequence and uploading the fasta file of each genome (Jolley et al. 2018).
The sequence reads were mapped to the PAO1-UW reference sequence (NC_002516) using Illumina's ELAND aligner (efficient large-scale alignment of nucleotide databases). ELAND aligns a large number of short reads to reference sequences, allowing up to two mismatches in the first 32 bases of a read.
sequence information and annotation for the complete set of predicted ORFs for this organism. Complete sequencing and analysis of the P. aeruginosa PA01 genome was completed and published in 2000 (Stover et al. 2000). The annotated genome predicted a coding capacity for 5570 ORFs, of which about half
