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13 paź 2022 · Sequencing of PAO1 revealed that P. aeruginosa has more regulatory genes than other sequenced bacteria genomes. The bacterium also has higher expression of transcriptional regulators in the LysR, AraC, ECF-σ, and two-component regulator families (Stover et al., 2000).
31 sie 2000 · Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of...
22 cze 2021 · We identified essential genes in LPAO using transposon mutant sequencing (Tn-seq) of pooled mutant libraries grown on LB agar and compared them to those found previously in PAO1 (see Materials and Methods).
12 mar 2024 · The pubMLST database was used to determine the multilocus sequence type, selecting Pseudomonas aeruginosa as an organism, single sequence and uploading the fasta file of each genome (Jolley et al. 2018).
The sequence reads were mapped to the PAO1-UW reference sequence (NC_002516) using Illumina's ELAND aligner (efficient large-scale alignment of nucleotide databases). ELAND aligns a large number of short reads to reference sequences, allowing up to two mismatches in the first 32 bases of a read.
6 sty 2020 · Up to October 2019, the Pseudomonas Genome Database, a database devoted to the information on Pseudomonas species , contained 4660 sequenced P. aeruginosa genomes, including 22 PAO1 sublines.
21 lip 2022 · RNA-seq analysis was performed on total RNA extracted from Pseudomonas aeruginosa PAO1 WT grown either in planktonic conditions for 5 h at 37°C, under shaking (200 rpm) in ABTG medium or grown for 72 h in biofilm flow cell chambers under continuous feeding of ABTG medium.
