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Basics of Pipetting Handbook. Your Guide to Getting the Most Out of Your Pipette. Table of Contents. Your pipette is a precision instrument. Master the basics of pipetting to get the best results from your assays through good pipetting techniques and practices.
The most commonly used function of electronic pipettes is the aliquoting of a reagent into multiple doses - multidispensing. By using a multichannel electronic pipette and repeat dispensing
The most commonly used function of electronic pipettes is the aliquoting of a reagent into multiple doses (stepper mode). By using a multichannel electronic pipette and stepper mode in microplate filling, the time used for filling the plate can be reduced from several minutes to less than a minute. Serological pipettes are used in
Use a graduated pipet when a wide range of volumes need to be measured. There are two main types of graduated pipets: Mohr pipet The Mohr pipet has graduations that end before the tip. It is calibrated “to deliver”, meaning that the last volume of liquid should remain in the tip. “To deliver” pipets are indicated with TD on the label.
INTRODUCTION TO MICRO-PIPETTING. Katherine Dorfman UMass Biology Department 2019. Goal for this lab: Master micropipetting technique. Introduction. bility to use a micropipettor. Micropipettors are used to make accurate measurements of extremely small volumes—from one milliliter down to.
GILSON GUIDE TO PIPETTING. Air-Displacement / Forward Mode. The forward mode is the standard way of pipetting with an air-displacement pipette like PIPETMAN. 1. Preparation. in a nearly vertical position. Depress the plunger smoot. Rest position. First stop. Second stop (purge) 2. Aspiration.
Pasteur pipettes (or pipets) are the most commonly used tool for transferring small volumes of liquids (< 5 mL 5 mL) from one container to another. They are considered disposable, although some institutions may clean and reuse them if they have a method for preventing the fragile tips from breaking.
