Search results
1 lis 2011 · Phenylethyl alcohol (PEA) agar allows growth of gram-positive organisms, particularly cocci, while inhibiting most gram-negative bacteria and fungi. Specifically, PEA agar is a selective medium that is used for the isolation of gram-positive Staphylococcus species and Streptococcus species from clinical specimens or specimens that contain ...
Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram-positive organisms, particularly cocci, from a sample containing a mixture of pathogens. The active ingredient, phenylethyl alcohol, inhibits or markedly reduces the growth of Gram-negative organisms by interfering with DNA synthesis.
Typically PEA agar is used to inhibit the common contaminants such as Escherichia coli and Proteus species. PEA agar may be prepared with and without 5% sheep blood supplement. PEA agar with 5% sheep blood is used to isolate most gram-positive and gram-negative anaerobes from enteric samples (7).
Lilley and Brewer developed Phenylethyl Alcohol Agar for isolation of gram-positive cocci, such as staphylococci and streptococci, from specimens containing mixed flora. 1 This medium permits the growth of gram-positive organisms while inhibiting most gram-negative
5 lip 2024 · Phenylethyl alcohol agar is formulated to create an environment conducive to the growth of specific bacterial species while suppressing others. The medium’s base typically consists of nutrient agar, which provides a rich source of nutrients necessary for bacterial growth.
1 lis 2011 · A phenylethyl alcohol (PEA) agar plate inoculated with a mixture of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria. Only growth of the gram-positive bacteria is seen on the PEA agar. When inoculated on tryptic soy agar plates, both bacterial types show growth.
Recommended Procedure. PP24 PEA Agar. Allow medium to reach room temperature. Using a direct inoculum from the specimen, perform a four-quadrant streak to obtain well-isolated colonies. A non-selective blood agar plate should also be inoculated to fully characterize the different types of organisms present in the sample.
