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1 lis 2011 · Phenylethyl alcohol (PEA) agar allows growth of gram-positive organisms, particularly cocci, while inhibiting most gram-negative bacteria and fungi. Specifically, PEA agar is a selective medium that is used for the isolation of gram-positive Staphylococcus species and Streptococcus species from clinical specimens or specimens that contain ...
15 maj 2024 · A selective medium called phenylethyl alcohol (PEA), is used to cultivate Gram positive organisms, especially cocci, in a sample containing pathogens. Phenylethyl alcohol is the active ingredient that inhibits or significantly reduces growth of Gram-negative organisms. It interferes with DNA synthesis.
Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram-positive organisms, particularly cocci, from a sample containing a mixture of pathogens. The active ingredient, phenylethyl alcohol, inhibits or markedly reduces the growth of Gram-negative organisms by interfering with DNA synthesis.
Incubation. Incubate plates for 24 to 48 hours at 35oC ± 2oC in an appropriate atmosphere. In some cases, a longer incubation, up to 1 week, may be needed. PEA blood agar plates can be incubated under aerobic, anaerobic, and 5% CO2 atmosphere based on the type of microorganisms being studied.
5 lip 2024 · Phenylethyl alcohol agar is formulated to create an environment conducive to the growth of specific bacterial species while suppressing others. The medium’s base typically consists of nutrient agar, which provides a rich source of nutrients necessary for bacterial growth.
SUMMARY AND EXPLANATION. Lilley and Brewer developed Phenylethyl Alcohol Agar for isolation of gram-positive cocci, such as staphylococci and streptococci, from specimens containing mixed flora.1 This medium permits the growth of gram-positive organisms while inhibiting most gram-negative organisms, especially the swarming of Proteus species.
Phenylethyl Alcohol Agar is formulated as per Lilley and Brewer (1) for the selective isolation of gram-positive bacteria. This medium can be supplemented with 5 % sheep blood.
