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26 sty 2019 · Phenol liquid. Phenol liquid is often used in molecular biology with trichloromethane and chloroform to separate RNA, DNA, or proteins, and isolate them in the pure form. This process is...
Phenol is a component in liquid–liquid phenol–chloroform extraction technique used in molecular biology for obtaining nucleic acids from tissues or cell culture samples. Depending on the pH of the solution either DNA or RNA can be extracted.
It should be noted that phenol is the simplest form, or parent compound, of the class of chemicals commonly referred to as phenols or phenolics, many of which are natural substances widely distributed throughout the environment.
From 1904, 5 per cent solutions of phenol (usually in vegetable oil) have been used to seal off varicose veins and to shrink haemorrhoids. 1 When such solutions are injected into a nerve, the cells and surrounding cells are destroyed and thus the ability to transmit pain.
Phenol dissolves to give a 9.3 percent solution in water, compared with a 3.6 percent solution for cyclohexanol in water. The association between water and phenol is unusually strong; when crystalline phenol is left out in a humid environment , it picks up enough water from the air to form liquid droplets.
Phenol acts as a weak acid. The hydroxyl group can release a proton (H +) from the oxygen atom, making phenol slightly acidic in aqueous solutions. The resulting equilibrium reaction in water can be represented as: [1-4] C 6 H 5 OH ⇌ C 6 H 5 O – + H + As a weak acid, phenol forms phenolate ions in solution.
Our initial focus in physiology is on the general body fluids that compose the three tissue compartment volumes of the body, those being: 1) Plasma (sometimes referred to as intravascular fluid); 2) Intracellular fluid (ICF); and 3) Extracellular fluid (ECF).
