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Northern Blot analysis is a reliable hybridization technique frequently used for the detection of a specific RNA transcript (e.g. mRNA) within a complex mixture.
HCR™ Northern Blot Technology Multiplexed quantitative RNA blotting. HCR™ probe sets, amplifiers, and buffers enable multiplexed quantification of RNA target size and abundance with automatic background suppression throughout the protocol.
Northern blot analysis reveals information about RNA identity, size, and abundance, allowing a deeper understanding of gene expression levels. Our Invitrogen™ portfolio comprises one of the industry’s most comprehensive product offerings for northern blot analysis.
We have established and validated Near Infrared (NIR) Fluorescence Northern Blot technology (Fig. 1). Our service includes sample preparation, RNA purification, probe synthesis, fluorescence labeling, Glyoxal-based RNA denaturation, detection system optimization, and data analysis.
Find northern blot probe and related products for scientific research at MilliporeSigma
20 wrz 2023 · The probes used on Northern blots are small RNA or DNA nucleotides. Traditionally, probes are labeled with radioisotopes, particularly 32 P, and detected using X-ray film or phosphor imaging on an instrument such as the new Azure Sapphire.
Probes. Probes for northern blotting are composed of nucleic acids with a complementary sequence to all or part of the RNA of interest. They can be DNA, RNA, or oligonucleotides with a minimum of 25 complementary bases to the target sequence. [5]
