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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity. Additional features include sgRNA Template Oligo Design and qPCR library quantification.
27 lip 2023 · Please use NEB’s Tm Calculator to determine the appropriate annealing temperature for your primer pair and NEB polymerase/buffer of interest. Unlike other calculators, the NEB Tm Calculator takes into consideration buffer components that affect melting temperatures and empirical observations when calculating the optimal annealing temperature.
The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair sequence, primer concentration, and DNA polymerase used in PCR. The calculator also calculates the primer length, percentage of GC content, molecular weight, and extinction coefficient.
Use this tool to estimate an appropriate incubation temperature when using NEB thermostable ligases and reaction buffers.
28 lis 2017 · Please use NEB’s Tm Calculator to determine the appropriate annealing temperature for your primer pair and polymerase/buffer of interest.
Choose a DNA, RNA, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry.
