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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
- NEBioCalculator
Choose a DNA, RNA, qPCR calculator from NEB, a leader in...
- NEBioCalculator
Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
Tm Calculator. Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
Tm Calculator. Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
Annealing temperatures can be optimized by doing a temperature gradient PCR starting 5°C below the calculated Tm. The NEB Tm Calculator is recommended to calculate an appropriate annealing temperature.
Use this tool to estimate an appropriate incubation temperature when using NEB thermostable ligases and reaction buffers.
Choose a DNA, RNA, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry.
