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Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
OligoAnalyzer™ Tool. Understand the expected properties of your oligos before you order them. Calculator for GC content, melting temperature (T m), molecular weight, extinction coefficient, µg/OD, nmol/OD, and more. Identify secondary structure potential. Minimize dimerization.
21 paź 2013 · Read this advice from our own thermodynamics specialist, Dr Richard Owczarzy, on the effects of probe and primer melting temperature (T m) on hybridization. He provides considerations for better oligo and PCR/qPCR assay design, including oligo concentration, salt, and base pairing mismatch positioning. Print Page.
Whether you are creating an assembly that incorporates multiple fragments, conducting your entire PCR at a single temperature, or incorporating intentionally mismatched nucleotides, careful primer design can set you up for success before you even pick up your pipette.
IDT offers a free online calculator, the OligoAnalyzer™ Tool, that will determine the T m of your oligonucleotide sequence for reaction conditions you specify. Be sure to include the Mg 2+ and dNTP concentrations you plan to use in your reaction, as this will affect the T m for your oligo.
3 paź 2024 · The IDT Primer Calculator is a helpful tool for researchers and scientists involved in molecular biology. It calculates the melting temperature (Tm) of a DNA primer, which is essential for designing efficient polymerase chain reaction (PCR) experiments.
We offer an easy-to-use Oligo Analyzer tool, OligoEvaluator™ that provides better primer dimer analysis values for PCR, and serves as an online oligonucleotide sequence calculator and Tm calculator.
