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Mueller-Hinton agar (MHA) is the best medium for routine antimicrobial susceptibility testing using the Kirby-Bauer disc diffusion method for non-fastidious bacteria (aerobe and facultative anaerobe).
Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.
Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton, who were microbiologists working at Harvard University.
27 maj 2024 · Antimicrobial susceptibility testing is a laboratory procedure performed by medical technologists (clinical laboratory scientists) to identify which antimicrobial regimen is effective for individual patients.
5 sty 2022 · Mueller Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing using the Kirby-Bauer disc diffusion method. MHA is recommended for the diffusion of antimicrobial agents impregnated on paper disc through an agar gel as described in CLSI Approved Standard.
This method consists of inoculating the isolated bacteria onto a Mueller-Hinton agar plate, followed by placing antibiotic-impregnated paper disks on the surface of the agar. By incubating this plate, antibiotics will diffuse into the agar in a gradient, the antibiotic concentration will decrease as the distance from the disk increases.
10 sie 2022 · The major use of Mueller Hinton Agar is for antimicrobial susceptibility testing. It has become the standard medium for the Bauer Kirby method and its performance is specified by the NCCLS. It can be used to cultivate Neisseria.
